Article
Assembly of an in vitro synthesized Escherichia coli outer membrane porin into its stable trimeric configuration.
The Journal of biological chemistry - 15 Mar 1990
de Cock H, Hendriks R, de Vrije T, Tommassen J
Abstract excerpt
The folding of in vitro synthesized outer membrane protein PhoE of Escherichia coli was studied in immunoprecipitation experiments with monoclonal antibodies which recognize cell surface-exposed conformational epitopes. The signal sequence appears to interfere with the formation of these conforma...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Hot Temperature
- Immunosorbent Techniques
- Macromolecular Substances
- Molecular Sequence Data
- Molecular Weight
- Mutation