Direct in situ structural analysis of recombinant outer membrane porins expressed in an OmpA-deficient mutant Escherichia coli strain.

Abstract excerpt

An OmpA-deficient mutant of an OmpF/OmpC-free Escherichia coli B strain was selected using phage K3. The mutant strain was characterized by SDS-gel electrophoresis, immunoblotting, and electron microscopy. All major outer membrane proteins, including OmpA, were absent. This strain was then transformed with the plasmid pMY222 encoding the K12 OmpF porin or with pBlue-script-derived plasmids, encoding the porins...

Topics

• Bacterial Outer Membrane Proteins
• Escherichia coli
• Gene Expression
• Genes, Bacterial
• Lipopolysaccharides
• Microscopy, Electron
• Molecular Structure
• Mutation
• Porins
• Recombinant Proteins