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Article

Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo.

1 Apr 1993

Abstract excerpt

Alkaline phosphatase (PhoA), localized in the periplasmic space of Escherichia coli, is a homodimeric metalloprotein containing two intramolecular disulfide bonds. We attempted to clarify the folding-assembly pathways of this enzyme by allowing in vitro-synthesized PhoA polypeptide to fold into active enzyme and by examining the occurrence of similar pathways in vivo by pulse-chase experiments. PhoA (lacking the...

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PMID: 8463326
DOI: 10.1016/s0021-9258(18)53073-8

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