Article
The assembly pathway of outer membrane protein PhoE of Escherichia coli.
European journal of biochemistry - 1 Jun 2000
Jansen C, Heutink M, Tommassen J, de Cock H
Abstract excerpt
The assembly of the wild-type and several mutant forms of the trimeric outer membrane porin PhoE of Escherichia coli was investigated in vitro and in vivo. In in vivo pulse-chase experiments, approximately half of the wild-type PhoE molecules assembled within the 30-s pulse in the native conformation in the cell envelope. The other half of the molecules followed slower kinetics, and three intermediates in this...
Topics
- Biochemistry
- Cell Membrane
- Dimethylamines
- Escherichia coli
- Escherichia coli Proteins
- Inclusion Bodies
- Mutation
- Porins
- Protein Folding