Article
Trimer-based design of pH-responsive protein cage results in soluble disassembled structures.
Biomacromolecules - 12 Sept 2011
Peng Tao, Lim Sierin
Abstract excerpt
Limited studies have been done on the interactions between subunits of self-assembling protein cages. E2 protein cage from Bacillus stearothermophilus was investigated in this work to impart pH-sensitive disassembly profile. Key amino acids were identified at the intratrimer and intertrimer interfaces, and histidine residues were introduced to these key sites to probe for their influences on the E2 assembly. We...
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