Article
Biogenesis of outer membrane protein PhoE of Escherichia coli. Evidence for multiple SecB-binding sites in the mature portion of the PhoE protein.
Journal of molecular biology - 20 Mar 1992
de Cock H, Overeem W, Tommassen J
Abstract excerpt
Efficient in vivo translocation of the precursor of Escherichia coli outer membrane protein PhoE across the inner membrane is shown to depend on SecB protein. A set of mutants, carrying internal deletions in the phoE gene, was used to locate a possible SecB-binding site and/or a site that makes t...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Binding Sites
- Biological Transport
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Porins
- Precipitin Tests