Article
A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes.
Journal of virology - 1 Mar 1990
Cosset F L, Legras C, Chebloune Y, Savatier P, Thoraval P, Thomas J L, Samarut J, Nigon V M, Verdier G
Abstract excerpt
An avian leukosis virus-based packaging cell line was constructed from the genome of the Rous-associated virus type 1. The gag, pol, and env genes were separated on two different plasmids; the packaging signal and the 3' long terminal repeat were removed. On a plasmid expressing the gag and pol g...
Topics
- Animals
- Avian Leukosis Virus
- Cell Line
- Chick Embryo
- Genes, Viral
- Genetic Complementation Test
- Genetic Engineering
- Genetic Vectors
- Helper Viruses
- Mutation
- Plasmids
- Restriction Mapping
- Transfection