Article
Preparation of biologically active platelet-derived growth factor isoforms AA and AB. Preferential formation of AB heterodimers.
European journal of biochemistry - 12 Jan 1990
Hoppe J, Weich H A, Eichner W, Tatje D
Abstract excerpt
We have expressed the mature platelet-derived growth factor (PDGF) A chain within a fusion protein of the cro repressor and beta-galactosidase in Escherichia coli. Monomeric PDGF-A was excised from this fusion protein by CNBr cleavage. After protection of thiols by S-sulfonation, this fragment wa...
Topics
- Amino Acid Sequence
- Animals
- Cell Division
- Cell Line
- Cloning, Molecular
- Coliphages
- Cyanogen Bromide
- Escherichia coli
- Genetic Vectors
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Platelet-Derived Growth Factor
- Protein Conformation
- Receptors, Cell Surface
- Receptors, Platelet-Derived Growth Factor