Identification of a conserved sequence in flavoproteins essential for the correct conformation and activity of the NADH oxidase NoxE of Lactococcus lactis.

Abstract excerpt

Water-forming NADH oxidases (encoded by noxE, nox2, or nox) are flavoproteins generally implicated in the aerobic survival of microaerophilic bacteria, such as lactic acid bacteria. However, some natural Lactococcus lactis strains produce an inactive NoxE. We examined the role of NoxE in the oxygen tolerance of L. lactis in the rich synthetic medium GM17. Inactivation of noxE suppressed 95% of NADH oxidase...

Topics

• Aerobiosis
• Amino Acid Sequence
• Conserved Sequence
• Fermentation
• Flavin-Adenine Dinucleotide
• Gene Expression Regulation, Bacterial
• Gene Expression Regulation, Enzymologic
• Lactococcus lactis
• Multienzyme Complexes
• Mutation
• NAD
• NADH, NADPH Oxidoreductases
• Oxygen