Article
Lambda Red-mediated synthesis of plasmid linear multimers in Escherichia coli K12.
Molecular & general genetics : MGG - 1 Sept 1990
Silberstein Z, Maor S, Berger I, Cohen A
Abstract excerpt
Expression of the red+ and gam+ genes of bacteriophage lambda in plasmids cloned in Escherichia coli wild-type cells leads to plasmid linear multimer (PLM) formation. In mutants that lack exonuclease I (sbcB sbcC), either of these lambda functions mediates PLM formation. In order to determine whe...
Topics
- Bacteriophage lambda
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Genes, Viral
- Kinetics
- Mutation
- Plasmids
- Recombination, Genetic
