Article
Lambda Red-mediated synthesis of plasmid linear multimers in Escherichia coli K12.
Molecular & general genetics : MGG - 1 Sept 1990
Silberstein Z, Maor S, Berger I, Cohen A
Abstract excerpt
Expression of the red+ and gam+ genes of bacteriophage lambda in plasmids cloned in Escherichia coli wild-type cells leads to plasmid linear multimer (PLM) formation. In mutants that lack exonuclease I (sbcB sbcC), either of these lambda functions mediates PLM formation. In order to determine whether PLM formation in sbcB sbcC mutants occurs by conservative (break-join) recombination of circular plasmids or by de...
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