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Article

Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.

Journal of molecular biology - 17 Jun 2011

Mathew Elizabeth, Bajaj Anshika, Connelly Sara M, Sargsyan Hasmik, Ding Fa-Xiang, Hajduczok Alexander G, Naider Fred, Dumont Mark E

Abstract excerpt

We describe a rapid method to probe for mutations in cell surface ligand-binding proteins that affect the environment of bound ligand. The method uses fluorescence-activated cell sorting to screen randomly mutated receptors for substitutions that alter the fluorescence emission spectrum of environmentally sensitive fluorescent ligands. When applied to the yeast α-factor receptor Ste2p, a G protein-coupled...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21477594
DOI: 10.1016/j.jmb.2011.03.059
PMCID: PMC3104124

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Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p.PMID 21477594Read ↗

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