Article
Performance evaluation of an in-house human immunodeficiency virus type-1 protease-reverse transcriptase genotyping assay in Cameroon.
Archives of virology - 1 Jul 2011
Fokam J, Salpini R, Santoro M M, Cento V, D'Arrigo R, Gori C, Perno C F, Colizzi V, Nanfack A, Gwom L C, Cappelli G, Takou D
Abstract excerpt
Most commercial HIV-1 genotyping assays are hampered by high cost in resource-limited settings. Moreover, their performance might be influenced over time by HIV genetic heterogeneity and evolution. An in-house genotyping protocol was developed, and its sequencing performance and reproducibility were compared to that of ViroSeq™. One hundred ninety plasma samples from HIV-1-infected subjects in Cameroon, a...
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