Article
Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.
Science (New York, N.Y.) - 31 Aug 1990
Raycroft L, Wu H Y, Lozano G
Abstract excerpt
The protein encoded by the wild-type p53 proto-oncogene has been shown to suppress transformation, whereas certain mutations that alter p53 become transformation competent. Fusion proteins between p53 and the GAL4 DNA binding domain were made to anchor p53 to a DNA target sequence and to allow measurement of transcriptional activation of a reporter plasmid. The wild-type p53 stimulated transcription in this...
Topics
- Base Sequence
- Cell Transformation, Neoplastic
- Gene Expression Regulation
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Oligonucleotide Probes
- Oncogene Proteins
- Phosphoproteins