Article
The Escherichia coli heat shock gene htpY: mutational analysis, cloning, sequencing, and transcriptional regulation.
Journal of bacteriology - 1 May 1993
Missiakas D, Georgopoulos C, Raina S
Abstract excerpt
We have identified a new heat shock gene, designated htpY, located 700 bp upstream of the dnaK dnaJ operon. We cloned it and showed that it is transcribed clockwise vis-à-vis the Escherichia coli genetic map, in the same direction as the dnaK dnaJ operon. The htpY gene encodes a 21,193-Da polypep...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Chromosome Mapping
- Cloning, Molecular
- Consensus Sequence
- DNA Mutational Analysis
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Heat-Shock Proteins
- Kanamycin Resistance
- Lac Operon
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Promoter Regions, Genetic
- RNA, Messenger