Article
[Scanning of c-kit gene mutations in acute myeloid leukemias using high-resolution melting analysis].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Jan 2011
Yao Dong-ming, Qian Jun, Lin Jiang, Chen Qin, Xiao Gao-fei, Wang Ya-li, Qian Zhen, Ji Run-bi, Li Yun, Yang Jing
Abstract excerpt
OBJECTIVE: To detect the common mutations (D816V and N822K) of c-kit gene in acute myeloid leukemia (AML) using high-resolution melting analysis (HRM). METHODS: HRM analysis was established to screen c-kit mutations in PCR products of c-kit exon 17 in 21 AML patients with t(8;21). PCR products were sequenced to confirm the mutation. RESULTS: HRM analysis identified an aberrant melting curve in 6 cases (28.6%),...
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