Article
Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis.
Genome biology - 1 Jan 2011
Ashelford Kevin, Eriksson Maria E, Allen Christopher M, D'Amore Rosalinda, Johansson Mikael, Gould Peter, Kay Suzanne, Millar Andrew J, Hall Neil, Hall Anthony
Abstract excerpt
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene...
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