Article
Forward Genetics by Sequencing EMS Variation-Induced Inbred Lines.
G3 (Bethesda, Md.) - 9 Feb 2017
Addo-Quaye Charles, Buescher Elizabeth, Best Norman, Chaikam Vijay, Baxter Ivan, Dilkes Brian P
Abstract excerpt
In order to leverage novel sequencing techniques for cloning genes in eukaryotic organisms with complex genomes, the false positive rate of variant discovery must be controlled for by experimental design and informatics. We sequenced five lines from three pedigrees of ethyl methanesulfonate (EMS)-mutagenized Sorghum bicolor, including a pedigree segregating a recessive dwarf mutant. Comparing the sequences of the...
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