Short reads and nonmodel species: exploring the complexities of next-generation sequence assembly and SNP discovery in the absence of a reference genome.

Abstract excerpt

How practical is gene and SNP discovery in a nonmodel species using short read sequences? Next-generation sequencing technologies are being applied to an increasing number of species with no reference genome. For nonmodel species, the cost, availability of existing genetic resources, genome complexity and the planned method of assembly must all be considered when selecting a sequencing platform. Our goal was to...

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