Article
The use of high-resolution melting analysis for genotyping Symbiodinium strains: a sensitive and fast approach.
Molecular ecology resources - 1 Mar 2011
Granados-Cifuentes Camila, Rodriguez-Lanetty Mauricio
Abstract excerpt
High-resolution melting (HRM) analysis is a closed-tube, rapid and sensitive technique able to detect DNA variations. It relies on the fluorescence melting curves that are obtained from the transition of double-stranded DNA (dsDNA) to single-stranded DNA (ssDNA) as a result of temperature increase. In this study, we evaluated the effectiveness of HRM as a tool to rapidly and precisely genotype monotypic...
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