Article
Use of regulated cell lysis in a lethal genetic selection in Escherichia coli: identification of the autoinducer-binding region of the LuxR protein from Vibrio fischeri ATCC 7744.
Journal of bacteriology - 1 Jul 1990
Shadel G S, Young R, Baldwin T O
Abstract excerpt
A lethal genetic selection utilizing the bacteriophage lambda lysis genes (S, R, RZ) has been developed and used in conjunction with a luminescence screen to allow the isolation and characterization of six missense mutations and two nonsense mutations in the luxR gene from Vibrio fischeri ATCC 77...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- Escherichia coli
- Genes, Lethal
- Hydroxylamine
- Hydroxylamines
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Repressor Proteins
- Restriction Mapping
- Trans-Activators
- Vibrio