Article
Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis.
Journal of bacteriology - 1 Jul 1990
Slock J, VanRiet D, Kolibachuk D, Greenberg E P
Abstract excerpt
Expression of Vibrio fischeri luminescence genes requires an inducer, termed autoinducer, and a positive regulatory element, the luxR gene product. A plasmid containing a tac promoter-controlled luxR was mutagenized in vitro with hydroxylamine, and luxR mutant plasmids were identified by their inability to complement a luxR deletion mutation in trans. Sixteen luxR mutant plasmids were obtained, ten of which...
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