Article
Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis.
Journal of bacteriology - 1 Jul 1990
Slock J, VanRiet D, Kolibachuk D, Greenberg E P
Abstract excerpt
Expression of Vibrio fischeri luminescence genes requires an inducer, termed autoinducer, and a positive regulatory element, the luxR gene product. A plasmid containing a tac promoter-controlled luxR was mutagenized in vitro with hydroxylamine, and luxR mutant plasmids were identified by their in...
Topics
- Bacterial Proteins
- Base Sequence
- Chromosome Mapping
- DNA, Bacterial
- Hydroxylamine
- Hydroxylamines
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Operon
- Phenotype
- Plasmids
- Repressor Proteins
- Trans-Activators
- Transcription, Genetic
- Vibrio