Article
Enhanced colorimetric detection on porous microarrays using in situ substrate production.
Analytical chemistry - 1 May 2011
Le Goff Gaelle C, Blum Loïc J, Marquette Christophe A
Abstract excerpt
A new technique is reported for the enhanced colorimetric detection of multiplexed hybridization onto porous membrane-based microarrays. This approach combines the use of horseradish peroxidase (HRP) as a label together with a chromogen substrate and a local production of the hydrogen peroxide required for substrate oxidation. This in situ production of coreagent is obtained using glucose oxidase (GOx) directly...
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