Article
Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli.
Journal of bacteriology - 1 May 1990
Lichens-Park A, Smith C L, Syvanen M
Abstract excerpt
Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic...
Topics
- Bacteriophage lambda
- Blotting, Southern
- Chromosomes, Bacterial
- Escherichia coli
- Genes, Viral
- Genotype
- Lysogeny
- Plasmids
- Rec A Recombinases
- Recombination, Genetic
- Restriction Mapping