Article
Development and characterization of a mouse cell line expressing the human V2 vasopressin receptor gene.
Molecular endocrinology (Baltimore, Md.) - 1 Feb 1990
Birnbaumer M, Hinrichs V, Themmen A P, Themen A P
Abstract excerpt
Human genomic DNA and the HSV tk gene were cotransfected into mouse Ltk- cells and assayed for the acquisition of a Gs-coupled receptor to obtain cell lines expressing human receptors that are so far unavailable. The transfected cells were distributed into 96-well microtitration plates at a density such that after HAT (100 microM hypoxanthine, 1 microM aminopterin, and 10 microM thymidine) selection each well...
Topics
- Adenylyl Cyclases
- Animals
- Arginine Vasopressin
- Cell Line
- Cell Line, Transformed
- Fibroblasts
- Gene Expression
- Ligands
- Mice
- Phenotype
- Receptors, Angiotensin