Mu gem3 as a tool to investigate the influence of chromosome supercoiling on gene expression in Escherichia coli K12.

doi:10.1016/0378-1097(90)90271-q

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Article

Mu gem3 as a tool to investigate the influence of chromosome supercoiling on gene expression in Escherichia coli K12.

FEMS microbiology letters - 1 Jan 1990

Bianchi E, Ruberti I, Ghelardini P, Paolozzi L

Abstract excerpt

We have developed a rapid method to investigate the influence of chromosome supercoiling on gene expression in Escherichia coli K12. This method exploits the ability of the gem3 mutant of the bacteriophage Mu, even in the prophagic state in immune cells, to induce relaxation of the host chromosom...

Topics

Bacteriophage mu
Cell Division
Chromosomes, Bacterial
DNA Replication
DNA, Bacterial
DNA, Superhelical
Escherichia coli
Gene Expression Regulation, Bacterial
Mutation

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