Article
Substitution of arginine for Leu444 in the reactive site of heparin cofactor II enhances the rate of thrombin inhibition.
The Journal of biological chemistry - 5 Apr 1990
Derechin V M, Blinder M A, Tollefsen D M
Abstract excerpt
Heparin cofactor II (HCII), a member of the "serpin" family of serine protease inhibitors, is a 65,600-Da plasma glycoprotein that inhibits thrombin and chymotrypsin. The rate of thrombin inhibition is stimulated approximately 1000-fold by heparin or dermatan sulfate. Thrombin and chymotrypsin cl...
Topics
- Amino Acid Sequence
- Arginine
- Base Sequence
- Binding Sites
- Chymotrypsin
- Dermatan Sulfate
- Escherichia coli
- Factor Xa Inhibitors
- Fibrinolysin
- Gene Expression
- Genetic Vectors
- Heparin
- Heparin Cofactor II
- Kallikreins
- Kinetics
- Leucine
- Molecular Sequence Data
- Mutation