Article
Contribution of basic residues of the A helix of heparin cofactor II to heparin- or dermatan sulfate-mediated thrombin inhibition.
FEBS letters - 3 Jul 2002
Hayakawa Yumiko, Hirashima Yutaka, Kurimoto Masanori, Hayashi Nakamasa, Hamada Hideo, Kuwayama Naoya, Endo Shunro
Abstract excerpt
Inhibition of thrombin by heparin cofactor II (HCII) is accelerated 1000-fold by heparin or dermatan sulfate. To investigate the contribution of basic residues of the A helix of HCII to this activation, we constructed amino acid substitutions (K101Q, R103L, and R106L) by site-directed mutagenesis. K101Q greatly reduced heparin cofactor activity and required a more than 10-fold higher concentration of dermatan...
Topics
- Amino Acid Sequence
- Amino Acids, Basic
- Binding, Competitive
- Dermatan Sulfate
- Genetic Variation
- Heparin
- Heparin Cofactor II
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Peptides
- Sepharose
- Serine Proteinase Inhibitors
- Thrombin