Cloning and characterization of a phospholipase gene from Erwinia chrysanthemi EC16.

doi:10.1111/j.1365-2958.1992.tb01999.x

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Article

Cloning and characterization of a phospholipase gene from Erwinia chrysanthemi EC16.

Molecular microbiology - 1 Jan 1992

Keen N T, Ridgway D, Boyd C

Abstract excerpt

A single gene (plcA) was cloned from a cosmid library of Erwinia chrysanthemi EC16 DNA that encoded an extracellular phospholipase. The gene was subcloned and DNA sequence data showed the presence of a single open reading frame encoding a protein with a predicted size of 39 kDa. The coding region...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Recombinant
Dickeya chrysanthemi
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression
Genes, Bacterial
Isoelectric Point
Molecular Sequence Data
Mutation
Phospholipases

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