Article
Cloning and characterization of a phospholipase gene from Erwinia chrysanthemi EC16.
Molecular microbiology - 1 Jan 1992
Keen N T, Ridgway D, Boyd C
Abstract excerpt
A single gene (plcA) was cloned from a cosmid library of Erwinia chrysanthemi EC16 DNA that encoded an extracellular phospholipase. The gene was subcloned and DNA sequence data showed the presence of a single open reading frame encoding a protein with a predicted size of 39 kDa. The coding region was G+C-rich and the protein had a predicted basic isoelectric point. The protein showed no significant homology with...
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