Article
Characterization of two cold-sensitive mutants of the beta-galactosidase from Lactobacillus delbruckii subsp. bulgaricus.
The Journal of biological chemistry - 25 Feb 1994
Adams R M, Yoast S, Mainzer S E, Moon K, Palombella A L, Estell D A, Power S D, Schmidt B F
Abstract excerpt
Methoxylamine mutagenesis of the beta-galactosidase gene from Lactobacillus delbrückii subsp. bulgaricus was used to generate cold-sensitive variants. Two variants, P429S and L317F, were characterized kinetically in order to determine the enzymatic consequences of these mutations. The kinetic par...
Topics
- Cold Temperature
- Hydrolysis
- Kinetics
- Lactobacillus
- Lactose
- Magnesium
- Molecular Weight
- Mutation
- beta-Galactosidase