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Article

Characterization of two cold-sensitive mutants of the beta-galactosidase from Lactobacillus delbruckii subsp. bulgaricus.

The Journal of biological chemistry - 25 Feb 1994

Adams R M, Yoast S, Mainzer S E, Moon K, Palombella A L, Estell D A, Power S D, Schmidt B F

Abstract excerpt

Methoxylamine mutagenesis of the beta-galactosidase gene from Lactobacillus delbrückii subsp. bulgaricus was used to generate cold-sensitive variants. Two variants, P429S and L317F, were characterized kinetically in order to determine the enzymatic consequences of these mutations. The kinetic parameters Km and Vmax on the synthetic substrate o-nitrophenyl-beta-D-galactopyranoside have been determined over a...

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PMID: 8119903

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