Article
Detection of minimal residual disease in acute lymphoblastic leukemia using immunoglobulin hypervariable region specific oligonucleotide probes.
Blood - 15 Nov 1990
Jonsson O G, Kitchens R L, Scott F C, Smith R G
Abstract excerpt
To develop a sensitive and specific assay for minimal residual disease in acute lymphoblastic leukemia (ALL), we exploited the enormous diversity of genomic sequences created by immune receptor gene rearrangements. To isolate clone-specific sequences, we first synthesized oligonucleotides that ma...
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- DNA
- Gene Amplification
- Gene Rearrangement
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Precursor Cell Lymphoblastic Leukemia-Lymphoma