Article
Analysis of B-lymphoid malignancies using allele-specific polymerase chain reaction: a technique for sequential quantitation of residual disease.
Blood - 1 Dec 1991
Billadeau D, Blackstadt M, Greipp P, Kyle R A, Oken M M, Kay N, Van Ness B
Abstract excerpt
The junctional sequences corresponding to the complementarity determining region 3 (CDR3) of rearranged heavy chain Ig genes can provide allele-specific markers in the detection of B-lymphoid malignancies. Consensus oligonucleotide primers were used to amplify CDR3 regions of rearranged heavy cha...
Topics
- Alleles
- B-Lymphocytes
- Base Sequence
- Bone Marrow
- Clone Cells
- DNA, Neoplasm
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Leukemia, B-Cell
- Molecular Sequence Data
- Multiple Myeloma
- Polymerase Chain Reaction