Article
Mutation detection with next-generation resequencing through a mediator genome.
PloS one - 31 Dec 2010
Wurtzel Omri, Dori-Bachash Mally, Pietrokovski Shmuel, Jurkevitch Edouard, Sorek Rotem
Abstract excerpt
The affordability of next generation sequencing (NGS) is transforming the field of mutation analysis in bacteria. The genetic basis for phenotype alteration can be identified directly by sequencing the entire genome of the mutant and comparing it to the wild-type (WT) genome, thus identifying acquired mutations. A major limitation for this approach is the need for an a-priori sequenced reference genome for the WT...
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