Article
Digital PCR: a tool in clostridial mutant selection and detection.
Applied microbiology and biotechnology - 1 Nov 2023
Dierick Evelien, Callens Chana, De Spiegelaere Ward, Ducatelle Richard, Van Immerseel Filip, Goossens Evy
Abstract excerpt
The ClosTron mutagenesis system has enabled researchers to efficiently edit the clostridial genome. Since site-specific insertion of the mobile ClosTron insert may cause errors, validation is key. In this paper we describe the use of digital PCR (dPCR) as an alternative tool in selecting clostridial mutant strains. Clostridium perfringens chitinase mutant strains were constructed in which the mobile ClosTron...
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