Article
A human NK cell activation/inhibition threshold allows small changes in the target cell surface phenotype to dramatically alter susceptibility to NK cells.
Journal of immunology (Baltimore, Md. : 1950) - 1 Feb 2011
Holmes Tim D, El-Sherbiny Yasser M, Davison Adam, Clough Sally L, Blair G Eric, Cook Graham P
Abstract excerpt
NK cell activation is negatively regulated by the expression of target cell MHC class I molecules. We show that this relationship is nonlinear due to an NK cell activation/inhibition threshold. Ewing's sarcoma family tumor cell monolayers, which were highly susceptible to NK cells in vitro, developed a highly resistant phenotype when cultured as three-dimensional multicellular tumor spheroid structures. This...
Topics
- Cell Line, Tumor
- Cell Membrane
- Cytotoxicity Tests, Immunologic
- Genetic Predisposition to Disease
- HeLa Cells
- Humans
- Immunity, Innate
- Immunophenotyping
- K562 Cells
- Killer Cells, Natural