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Varicella-Zoster Virus Transcriptome in Latently Infected Human Ganglia

23 Dec 2010

Abstract excerpt

We recently developed a novel multiplex reverse transcription (RT)-PCR assay that allows rapid and sensitive detection of transcripts corresponding to all 68 unique varicella-zoster virus (VZV) open reading frames (ORFs) in only five amplification reactions (M. A. Nagel, D. Gilden, T. Shade, B. Gao, and R. J. Cohrs, J. Virol. Methods 157:62-68, 2009). Herein, we applied multiplex RT-PCR analysis to mRNA extracted...

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PMID: 21177814
DOI: 10.1128/jvi.01862-10

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