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Linear and Circular Plasmid Content in Borrelia burgdorferi Clinical Isolates

21 Jun 2003

Abstract excerpt

The genome of Borrelia burgdorferi, the etiologic agent of Lyme disease, is composed of a linear chromosome and more than 20 linear and circular plasmids. Typically, plasmid content analysis has been carried out by pulsed-field gel electrophoresis and confirmed by Southern hybridization. However, multiple plasmids of virtually identical sizes (e.g., lp28 and cp32) complicate the interpretation of such data. The...

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PMID: 12819050
DOI: 10.1128/iai.71.7.3699-3706.2003

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