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Article

A cell-free microtiter plate screen for improved [FeFe] hydrogenases.

PloS one - 10 May 2010

Stapleton James A, Swartz James R

Abstract excerpt

BACKGROUND: [FeFe] hydrogenase enzymes catalyze the production and dissociation of H(2), a potential renewable fuel. Attempts to exploit these catalysts in engineered systems have been hindered by the biotechnologically inconvenient properties of the natural enzymes, including their extreme oxygen sensitivity. Directed evolution has been used to improve the characteristics of a range of natural catalysts, but has...

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PMID: 20479937
DOI: 10.1371/journal.pone.0010554
PMCID: PMC2866662

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