Identification of cis-acting sequences required for translational autoregulation of the ermC methylase.

Abstract excerpt

ermC methylase gene expression has been shown to be limited by translational autorepression, presumably due to methylase binding to ermC mRNA. It was found that this repression occurs in trans, yielding a 50% reduction in translation of an ermC-lacZ fusion mRNA. We investigated the ermC mRNA sequences required for translational repression in vivo. A series of deletions identified sequences in the 5' regulatory...

Topics

• Bacillus subtilis
• Base Sequence
• DNA, Bacterial
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Homeostasis
• Methyltransferases
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Oligonucleotide Probes