A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms.

Abstract excerpt

BACKGROUND: Current techniques of screening bacterial artificial chromosome (BAC) libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chromosome Mapping
• Share to Chromosomes, Artificial, Bacterial