Article
Sequencing-based variant detection in the polyploid crop oilseed rape.
BMC plant biology - 6 Aug 2013
Wells Rachel, Trick Martin, Fraser Fiona, Soumpourou Eleni, Clissold Leah, Morgan Colin, Pauquet Jérôme, Bancroft Ian
Abstract excerpt
BACKGROUND: The detection and exploitation of genetic variation underpins crop improvement. However, the polyploid nature of the genomes of many of our most important crops represents a barrier, particularly for the analysis of variation within genes. To overcome this, we aimed to develop methodologies based on amplicon sequencing that involve the incorporation of barcoded amplification tags (BATs) into PCR...
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