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Article

Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Chemical communications (Cambridge, England) - 28 Feb 2011

Hanes Jeremiah W, Chatterjee Debashree, Soriano Erika V, Ealick Steven E, Begley Tadhg P

Abstract excerpt

This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 21125117
DOI: 10.1039/c0cc04228e
PMCID: PMC3776480

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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