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Article

A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detection and genotyping in plants.

Journal of integrative plant biology - 1 Dec 2010

Li Yi-Dan, Chu Zhi-Zhan, Liu Xiang-Guo, Jing Hai-Chun, Liu Yao-Guang, Hao Dong-Yun

Abstract excerpt

High-resolution melting (HRM) analysis relies on the use of fluorescent dyes, such as LCGreen, ResoLight, and SYTO9, which bind in a saturated manner to the double-stranded DNAs. These dyes are expensive in use and may not be affordable when dealing with a large quantity of samples. EvaGreen is a much cheaper DNA helix intercalating dye and has been used in quantitative real-time polymerase chain reaction (PCR)...

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PMID: 21106003
DOI: 10.1111/j.1744-7909.2010.01001.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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