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Article

High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Sept 2009

Hofinger Bernhard J, Jing Hai-Chun, Hammond-Kosack Kim E, Kanyuka Kostya

Abstract excerpt

An original method has been established for the identification of novel alleles of eukaryotic translation initiation factor 4E (eIF4E) gene, which is required for resistance to agronomically important bymoviruses, in barley germplasm. This method involves scanning for sequence variations in cDNA-derived PCR amplicons using High-resolution melting (HRM) followed by direct Sanger sequencing of only those amplicons...

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PMID: 19578831
DOI: 10.1007/s00122-009-1094-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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