Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genetic screening for bacterial mutants in liquid growth media by fluorescence-activated cell sorting.

Journal of microbiological methods - 1 Jan 2011

Abuaita Basel H, Withey Jeffrey H

Abstract excerpt

Many bacterial pathogens have defined in vitro virulence inducing conditions in liquid media which lead to production of virulence factors important during an infection. Identifying mutants that no longer respond to virulence inducing conditions will increase our understanding of bacterial pathogenesis. However, traditional genetic screens require growth on solid media. Bacteria in a single colony are in every...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Culture Media
Share to Flow Cytometry
Share to Genetic Testing

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetics, Microbial

Share to Mutation

Share to Vibrio cholerae

Share to Virulence Factors

Source

Open in PubMed Open full text

Identifiers

PMID: 21094189
DOI: 10.1016/j.mimet.2010.11.003
PMCID: PMC3026434

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genetic screening for bacterial mutants in liquid growth media by fluorescence-activated cell sorting.PMID 21094189Read ↗

Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus.Semantic proximity Read ↗

Tools for Rapid Genetic Engineering of Vibrio fischeri.Semantic proximity Read ↗

A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae.Semantic proximity Read ↗

A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide.Semantic proximity Read ↗

Droplet Tn-Seq combines microfluidics with Tn-Seq for identifying complex single-cell phenotypes.Semantic proximity Read ↗

Site-directed mutagenesis and gene deletion using reverse genetics.Semantic proximity Read ↗

Effects of flaC Mutation on Stringent Response-Mediated Bacterial Growth, Toxin Production, and Motility in Vibrio cholerae.Semantic proximity Read ↗

Chemical genomics screening reveals novel functions for unannotated genes in <i>Vibrio cholerae</i> C6706Semantic proximity Read ↗

Chemical tools for dissecting bacterial physiology and virulence.Semantic proximity Read ↗

Deployment of a Vibrio cholerae ordered transposon mutant library in a quorum-competent genetic background.Semantic proximity Read ↗

Rapid ordering of barcoded transposon insertion libraries of anaerobic bacteriaSemantic proximity Read ↗

Identification of Salmonella enterica serovar Enteritidis mutants overproducing OMVs as vaccine candidates.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.