Article
Fluorogenic ribonuclease protection (FRIP) analysis of single nucleotide polymorphisms (SNPs) in Japanese Rice (Oryza sativa L.) DNA for cultivar discrimination.
Bioscience, biotechnology, and biochemistry - 1 Jan 2010
Kitaoka Momoko, Wada Takuya, Nishio Takeshi, Goto Masahiro
Abstract excerpt
A rapid and easy method to discriminate plant cultivars is indispensable to confirm food labeling. We established a fluorogenic ribonuclease protection (FRIP) assay to discriminate Japanese rice (Oryza sativa L.) cultivars based on single nucleotide polymorphisms (SNPs). The FRIP assay uses a hybridization technique between fluorescent probes and the target sequence prepared by run-off transcription, but the...
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