Article
Detection of SNPs in fish DNA: application of the fluorogenic ribonuclease protection (FRIP) assay for the authentication of food contents.
Journal of agricultural and food chemistry - 13 Aug 2008
Kitaoka Momoko, Okamura Nobuko, Ichinose Hirofumi, Goto Masahiro
Abstract excerpt
The fluorogenic ribonuclease protection (FRIP) assay was used to detect single nucleotide polymorphisms (SNPs) in commercially produced fish products. By using fluorescence resonance energy transfer (FRET) between fluorophore and quencher labeled probes, the species-specific cleavage of sample RNA was detected by measuring the fluorescence intensity during the FRIP assay. We were able to discriminate raw and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
