Article
Identification of WA-type three-line hybrid rice with real-time polymerase chain reaction (PCR) method.
Applied biochemistry and biotechnology - 1 Feb 2012
Cheng Y, Gao B D, Chen H Y, Mao J J, Cao A X, Zhu J G, Zhu S F
Abstract excerpt
A real-time fluorescent PCR (RTF-PCR) was developed to detect and quantify wild abortive (WA)-type three-line hybrid rice (Oryza sativa L.). The mitochondrial R₂₋₆₃₀ WA gene was reported to be closely related to male sterility in plants, and developed as a molecular maker to identify the cytoplasmic male sterility system of hybrid rice. First, we got the DNA sequence of R₂₋₆₃₀ WA gene in 17 rice species with...
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