Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

cDNA cloning of nine S alleles and establishment of a PCR-RFLP system for genotyping European pear cultivars.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 May 2006

Takasaki T, Moriya Y, Okada K, Yamamoto K, Iwanami H, Bessho H, Nakanishi T

Abstract excerpt

Nine full-length cDNAs of S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars by RT-PCR and 3' and 5' RACE. Comparison of the nucleotide sequences between the nine S-RNases cloned and 13 putative S alleles previously amplified by genomic PCRs revealed that seven corresponded to Sa, Sb, Sd, Se, Sh, Sk and Sl alleles, and the other two were new S alleles (designated as Sq and Sr...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Conserved Sequence

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA, Complementary

Share to DNA, Plant

Share to Europe

Share to Polymorphism, Restriction Fragment Length

Share to Sequence Homology, Amino Acid

Source

Identifiers

PMID: 16565843
DOI: 10.1007/s00122-006-0257-7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

cDNA cloning of nine S alleles and establishment of a PCR-RFLP system for genotyping European pear cultivars.PMID 16565843Read ↗

Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles.Semantic proximity Read ↗

Determination of S-genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses.Semantic proximity Read ↗

Molecular identification of two new self-incompatible alleles (S-alleles) in Chinese pear (Pyrus bretschneideri).Semantic proximity Read ↗

Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility.Semantic proximity Read ↗

Pistil-function breakdown in a new S-allele of European pear, S21*, confers self-compatibility.Semantic proximity Read ↗

Molecular cloning and nucleotide sequences of cDNAs encoding S-allele specific stylar RNases in a self-incompatible cultivar and its self-compatible mutant of Japanese pear, Pyrus pyrifolia Nakai.Semantic proximity Read ↗

Deletion of a 236 kb region around S 4-RNase in a stylar-part mutant S 4sm-haplotype of Japanese pear.Semantic proximity Read ↗

Self-incompatibility (S) alleles of the Rosaceae encode members of a distinct class of the T2/S ribonuclease superfamily.Semantic proximity Read ↗

Two neutral variants segregating at the gametophytic self-incompatibility locus of European pear (Pyrus communis L.) (Rosaceae, Pyrinae).Semantic proximity Read ↗

S-Locus Genotyping in Japanese Plum by High Throughput Sequencing Using a Synthetic S-Loci Reference Sequence.Semantic proximity Read ↗

Polymorphism of SFBB-gamma and its use for S genotyping in Japanese pear (Pyrus pyrifolia).Semantic proximity Read ↗

Allelic diversity of S-RNase alleles in diploid potato species.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.