Article
Cloning and mapping of variety-specific rice genomic DNA sequences: amplified fragment length polymorphisms (AFLP) from silver-stained polyacrylamide gels.
Genome - 1 Apr 1996
Cho Y G, Blair M W, Panaud O, McCouch S R
Abstract excerpt
An efficient technique for cloning DNA from silver-stained denaturing polyacrylamide gels was developed to allow the isolation of specific bands obtained from selective restriction fragment amplification (SRFA). This method proved as reliable as cloning radioactively labelled SRFA bands from the...
Topics
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Coloring Agents
- DNA Primers
- DNA, Plant
- Electrophoresis, Polyacrylamide Gel
- Gene Amplification
- Genetic Variation
- Molecular Sequence Data
- Oryza
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Silver