Article
Developmental regulation of IgM secretion: the role of the carboxy-terminal cysteine.
Cell - 9 Mar 1990
Sitia R, Neuberger M, Alberini C, Bet P, Fra A, Valetti C, Williams G, Milstein C
Abstract excerpt
B lymphocytes do not secrete IgM, and plasma cells only secrete IgM polymers. Here we show that both events are attributable to the tailpiece found at the carboxyl terminus of mus chains, and we specifically implicate Cys-575. Thus, if Cys-575 was mutated, IgM was secreted by B cells. Similarly, a mutant IgG containing a mus tailpiece became largely retained within the cell; secretion was restored upon mutation...
Topics
- Amino Acid Sequence
- Animals
- B-Lymphocytes
- Base Sequence
- Cell Line
- Cysteine
- Humans
- Immunoglobulin M
- Immunoglobulin mu-Chains
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes